应用领域：制药/化妆品,生物制药/仿制药
检测样品：重组人生长激素
检测项目：反相分离
参考标准：无
方案摘要
Column: ChromCore 1000 C18-T, 5 μm
Dimension: 4.6×250 mm
Mobile Phase: A) 40/60 v/v MeCN/200 mM (NH4)2SO4 in 80 mM
NaH2PO4 solution containing 0.5% perchloric acid
B) 80/20 v/v MeCN/H2O
Gradient: t (min) %A %B
0 79 21
60 74 26
61 79 21
72 79 21
Flow Rate: 1.0 mL/min
Temperature: 45 °C
Injection: 20 μL
Detection: UV 215 nm
Sample: 重组人生长激素
Peak: 1. 重组人生长激素
