应用领域：制药/化妆品,生物制药/仿制药
检测样品：双抗电荷异质体
检测项目：离子交换
参考标准：无
方案摘要
Column: BioCore WCX, 5 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM PB, pH6.5
B) 300 mM NaCl in 20 mM PB, pH6.5
Gradient: t (min) %A %B
-15 90 10
0 90 10
0.1 90 10
20 75 25
20.1 0 100
23 0 100
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 5 μL
Detection: UV 280 nm
Sample: 双抗 (~5.0 mg/mL in Mobile Phase A)
