应用领域：制药/化妆品,生物制药/仿制药
检测样品：半胱氨酸偶联单抗药物
检测项目：疏水作用
参考标准：无
方案摘要
Column: BioCore HIC-Butyl, 5 μm
Dimension: 4.6×100 mm
Mobile Phase : A) 2.0 M (NH4)2SO4 in 100 mM phosphate buffer, pH7.0
B) 100 mM phosphate buffer, pH7.0
C) Isopropanol
Gradient: t(min) %A %B %C
-10 75 25 0
0 75 25 0
1 75 25 0
15 0 75 25
20 0 75 25
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 10 μL
Detection: UV 280 nm
Sample: Cysteine conjugated ADC (~1 mg/mL in Mobile Phase A)
