应用领域：制药/化妆品,生物制药/仿制药
检测样品：Fab and Fab-PEG
检测项目：离子交换
参考标准：无
方案摘要
Column: BioCore WCX, 10 μm
Dimension: 4.6×150 mm
Mobile Phase: A) 20 mM MES, pH5.5
B) 300 mM NaCl in 20 mM MES, pH5.5
Gradient: t(min) %A %B
-10 100 0
0 100 0
20 60 40
20.1 0 100
25 0 100
Flow Rate: 1.0 mL/min
Temperature: 30 °C
Injection: 2 μL
Detection: UV 280 nm
Sample: Fab-PEG (3 mg/mL)
Fab (5 mg/mL)
