This application shows the advantages of using the Thermo Scientific™ Accucore™ Vanquish™ C18 1.5 μm UHPLC column and the Vanquish UHPLC system for the separation of 18 herbicides. The advanced capabilities of the Vanquish UHPLC system allow the Accucore Vanquish columns to be operated at higher flow rates that enable development of rapid, high-performance analytical methods. Comparisons are made with other commercially available sub-2 μm solid core columns, demonstrating improved peak capacity and resolution.