To demonstrate how the Thermo Scientific™ Vanquish™ Diode Array Detector (DAD) HL with a 60 mm high-sensitivity LightPipe™ flow cell boosts UV trace detection performance of HPLC applications. Detection side effects such as refractive index effects and peak dispersion are explained and used to evaluate the data from this flow cell. The peak dispersion data further leads to recommendations which column formats are best suited for this type of flow cell. The Vanquish DAD is available for the Vanquish system platform and the Thermo Scientific™ Dionex™ UltiMate™ 3000 UHPLC system platform.