质谱已广泛应用于科研、制药、医疗、食品、环境等各个领域。虽然质谱仪本身的扫描速度可以达到 10-1000 Hz，但目前通用的质谱进样分离手段与质谱仪的高扫描速度并不匹配，从而大大限制了质谱分析的通量。为了解决上述问题，SCIEX开发了声波激发与质谱联用系统 AEMS（Acoustic Ejection Mass Spectrometry）技术。这一革命性的创新科技通过开放端口接口（Open Port Interface, OPI）将高速声波液滴激发技术（Acoustic Droplet Ejection, ADE）与经典的 ESI 电离方法有机的结合在一起，同时实现零交叉污染、精准定量的高通量分析平台。

SCIEX 自2020年正式推出超高通量声波激发质谱 Echo™ MS系统后，获得了广泛关注和高度评价，全球累计销量已超过50多台。目前Echo™ MS广泛应用于化合物的高通量筛选、药物的药代动力学分析、合成生物学、高通量靶向组学研究等领域。

SCIEX Echo™MS 主要应用方向

 

SCIEX Echo™MS 工作原理

 

SCIEX Echo MS 全球典型用户

 

SCIEX Echo™MS 新品预告

 

SCIEX将于2024年2月美国SLAS会议 (Society for Laboratory Automation & Screening) 发布新一代Echo产品， 敬请期待！

 

参考文献

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Pfizer. Ultra-high-throughput acoustic droplet ejection-open port interface-mass spectrometry for parallel medicinal chemistry, ACS Med. Chem. Lett. 2020, 11, 6, 1101–1110.

BI. Ultrahigh-throughput ESI-MS: sampling pushed to six samples per second by acoustic ejection mass spectrometry, Anal. Chem. 2020, 92, 18, 12242–12249.

BMS. Ultrahigh-throughput and chromatography-free bioanalysis of polar analytes with acoustic ejection mass spectrometry, Anal. Chem. 2020, 92, 19, 13525–13531.

BMS. Acoustic ejection/full-scan mass spectrometry analysis for high-throughput compound quality control, SLAS Technol. 2021, 26, 178-188.

Merck. Direct analysis from phase-separated liquid samples using ADE-OPI-MS: Applicability to high-throughput screening for inhibitors of diacylglycerol acyltransferase 2, Anal. Chem. 2021, 93, 15, 6071–6079.

BI. Acoustic ejection mass spectrometry: a fully automatable technology for high-throughput screening in drug discovery, SLAS Discov. 2021, 26, 961-973.

BMS. A full scan data review tool to match the speed of acoustic ejection mass spectrometry, LCGC North America, 2022, 40, 7, 314-320.

University of Tokyo, Application of acoustic ejection MS system to high-throughput screening for SARS-CoV-2 3CL protease inhibitors, Chem. Pharm. Bull., 2022, 70, 199-201.

BMS. Acoustic ejection mass spectrometry: Development, applications, and future perspective, Biomed Chromatogr. 2022, 26, e5278.

Merck. Ultrahigh-throughput intact protein analysis with acoustic ejection mass spectrometry, J. Am. Soc. Mass Spectrom. 2023, 34, 1, 4–9.

BI. Label-free high-throughput screening via acoustic ejection mass spectrometry put into practice, SLAS Discov. 2023, 28, 5, 240-246.

IOCB. Discovery and structural characterization of monkeypox virus methyltransferase VP39 inhibitors reveal similarities to SARS-CoV-2 nsp14 methyltransferase, Nature Commun. 2023, 14, 1, 2259.

IOCB. Rational design of highly potent SARS-CoV-2 nsp14 methyltransferase inhibitors, ACS Omega, 8, 30, 27410-27418.

St. Jude. A high-throughput quality control method for assessing the serial dilution performance of dose–response plates with acoustic ejection mass spectrometry, SLAS Technol. 2023, in press.

IOCB. Inhibition of FLT3-ITD kinase in acute myeloid leukemia by new imidazo[1,2-b]pyridazine derivatives identified by scaffold hopping, J. Med. Chem. 66, 16, 11133-11157.

In Ovo. High throughput mass spectrometry assay for early chick gender determination: less than 3s total analysis time per sample, ChemRxiv. 2023, DOI: 10.26434/chemrxiv-2023-8z9gp

Merck. Accelerating pharmaceutical process development with an acoustic droplet ejection-multiple reaction monitoring-mass spectrometry workflow, Anal. Chem. 2024, in press.

领域：生理生态,分子生物学,蛋白/抗体/蛋白质组,其他