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针对已知基因组物种，通过群体内pooling建库的方法，进行全基因组重测序（20X以上/群体），采用生物信息学方法全基因组范围内扫描变异位点，检测性状相关的基因区域及其功能基因，可大大加速重要分子标记的发现，进而为后续分子育种提供可靠依据。

 

技术路线

适用范围

基因组已知的物种，具有明显性状差异的群体，每个群体不少于10个个体。

产品优势

目标明确—挖掘重要性状关键功能基因；

成本降低—群体内pooling测序；

利用率高—可利用已发表物种的数据库信息；

高效精准—完成高密度的变异检测（SNP）；

 

精确定位—缩小定位群体候选基因范围

                                                 基因区段及变异分析

                                                               T-DNA插入位点检测

●参考文献

 

1、Erik A, Abhirami R, Maja A, et al. The genomic signature of dog domestication reveals adaptation to a starch-rich diet. Nature, 2013, 495, 360–364.
2、Rubin CJ, Zody MC, Eriksson J, et al. Whole-genome resequencing reveals loci under selection during chicken domestication. Nature, 2010, 464: 587-591.