MetaFluor 离子成像系统 

Molecular Devices 的离子成像系统，能够提供整个系统和系统升级服务。系统内包含的高精度和高速度部件能够为高速度离子成像分析和高精度比例成像,配合高水平的 MetaFluor 软件系统，能够用于：

1，神经细胞，心肌细胞，骨骼肌细胞，胰岛细胞等

2，各种离子 (Ca²⁺、Mg²⁺、Zn²⁺、K⁺、Cl^-、Na⁺、pH) 的荧光比值测量及单波长荧光测量

3，快速的 FRET 测量

光源系统

高精度高稳定性的光源系统，能够确保稳定可靠的离子浓度测量，同时提供超长寿命，无需频繁更换灯泡，更低使用成本。

波长切换

高速和超高速的切换装置，能够快速切换激发光波长，方便快速地进行比例和非比例成像分析。

成像检测器

MetaFluor 比例成像系统配置了针对不用实验要求的优化 sCMOS 检测器，提供高分辨率、高速度、高灵敏度的实验结果。

能够整合多种外围设备

MetaFluor 比例成像系统能够整合多种外周设备，包括与膜片钳联用，整合自动灌流设备等。并能通过自动控制外围设备实现实验无人值守自动化。

实时获得细胞离子绝对浓度

系统能够无限制圈选感兴趣区域 (ROI) 与细胞，能够在图像采集的过程中实时获得荧光亮度曲线、比例亮度曲线和离子浓度绝度值。

实验自动化

1，能够通过 Journal 功能自行设计和更改实验流程，实现实验流程自动化。

2，通过 Journal 在实验任意步骤控制外设进行细胞刺激，灌流的开关等，并将流程自动化。

3，对于 Indo 1 和 FRET 实验，可以进行双发射通道同时成像和分析。

超过 400 个客户的成功案例

1，MetaFluor 系统进行离子成像文章超过 400 篇，成为离子成像应用的优质系统。

同时展示多波长图像和自定义的测量曲线。当对之前的图像进行二次分析时，通过鼠标点击曲线任意位置能够快速展示出与之相对应的图像。CHO 细胞标记 Fura-2。

图像来源：the Biomedical Sciences short course,Marine Biological Laboratory, Woods Hole,MA. Courtesy of Lynda Pierini, PhD,Cornell Medical Center, Ken Dunn, PhD,Indiana University-Purdue University,and Professor Colin Izzard, SUNY University of Albany.

MetaFluor成像系统主要组成及特点

MetaFluor

Fluorescence Ratio Imaging

Fluorescence ratio imaging is the monitoring of live cells in which a fluorescent indicator of intracellular ions is introduced. Indicator dyes have been designed to shift their fluorescence excitation or emission spectrum when binding with specific ions. Images are obtained at two different wavelengths, typically matching the absorption bands at the high and low binding conditions. 

 By ratioing the intensities in the images, it is possible to construct a map showing the local ion concentrations throughout the field of view. Since the monitoring process is nondestructive, image acquisition can be repeated frequently to trace and monitor the time course of cellular responses.

The MetaFluor® Imaging System is designed for dual-wavelength intracellular ion measurements. The system provides simultaneous display of the raw data, ratio image, graphs of intensities, ratios and ion concentrations, and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured simultaneously.

Custom Configuration

Toolbars, menus, wizards and dialog boxes help move you through the image processing steps quickly. Features such as multiple image windows, flexible device control, synchronization and timing, and journals allow for automated image acquisition and analysis unlike any other system.

With MetaFluor, you customize the set-up once, then let the experiment run by itself. You are able to collect a large amount of data online and process it with either MetaFluor or an analysis-only copy of the software.