Human RTK Phosphorylation Antibody Array 1 Analysis Tool
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Human RTK Phosphorylation Antibody Array 1 Analysis Tool

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Antibody Arrays and Their Applications

1.Array Product Portfolio
Cytokine Antibody Arrays for
Human, Mouse and Rat samples
Quantitative and semi-quantitative
ELISA Sandwich and Label-based

Phosphorylation Antibody Arrays
RTK and EGFR

Antibody Array Analysis software
Protein Arrays

2.Array using protein as a capture molecule
Recombinant/Purified Protein
Antibody
Peptide arrays
Reversed phase (biological samples)
Transcription factor
Tissue arrays


3.Types of Cytokine Array Targets

Cytokines
Inflammatory factors (interleukins, etc.)
Chemokines
Angiogenesis
Atherosclerosis
MMPs
Growth Factors
Adipokines
Soluble receptor
Signal transduction (Phosphorylation)
Broad Screen (all of the above)

4.全球领先优势:

(1)Global analysis of gene or protein expression
(2)High-throughput assays
(3)Substantially reduced costs
(4)Small sample volume

5.Antibody Arrays vs. Mass Spec

(1)“Unbiased” = abundant
Nanogram/ml vs. picogram/ml sensitivity
(2)Cheaper per sample
(3)Faster throughput
(4)Speeds up discovery process: Discovery
Validation
Application
All in one platform

6.Antibody Arrays vs. Luminex

(1)Affixing capture Ab reduces interactions
(2)Can screen hundreds of proteins
(3)More flexibility
Equipment
Mode of detection (Fluor, Chemi, Color)
(4)Less equipment maintenance


7.cDNA Arrays vs. Antibody Arrays

(1)cDNA arrays measure only RNA levels
(2)↑ RNA ≠↑protein levels or function
(3)Protein functions are modulated by
Interactions with other proteins, DNA and/or RNA
Biochemical modification of individual amino acids
(4)Antibody arrays measure protein levels
Can demonstrate patterns of coupled protein expression
Can detect biochemical modifications of protein
Can be used to detect protein-protein, protein-DNA or protein-RNA interactions
Results are more biologically meaningful

8.Formats

(1)Membrane
Chemiluminescence detection
(2)Glass Slides
Fluorescence detection
(3)96-Well Plate
(4)Sandwich ELISA Array
(5)Label-based Array
(6)Quantitative vs. Semi-Quantitative

9.Features of Cytokine Antibody Array (membrane-based):

(1)Detection
Up to 274 cytokines in one experiment.
Detection in picogram(pg) range.
(2)Highly specific, little cross-reactivity
(3)Handles a wide range of samples
Conditioned media, plasma, serum , urine and other body fluids
Cell or tissue lysates
(4)Requires low sample volumes
As little as 100 μLof sample
(5)Easy to use, requires no specialized equipment
(6)Cost-effective for routine use

10.Advantages of Cytokine Array System: G-Series

(1)Requires laser fluorescence scanner
(2)Sensitivity similar to membrane arrays
(3)Greater dynamic range
(4)Cheaper per sample than membranes
(5)High-throughput (100’s of samples/day)
(6)Small sample volume (10-50 μL/array)


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