The ADDA High Speed Dual Arm Autosampling System

           A high-speed, dual-arm autosampler that provides ADME discovery scientists with the fastest high-quality sample delivery available for traditional LC/MS.  At under 10 seconds per sample in trap/elute mode, and with up to 8 unique injection ports, the ADDA system is designed for the high-throughput lab.  With the ADDA system, large volumes of samples can be analyzed in-house with extraordinary turnaround time and reliability.

Multiple autosamplers in one

The system achieves its speed and flexibility with 2 high-speed, highaccuracy robotic arms, each equipped with a needle and up to four injection ports.  Batches can be submitted to use either one arm or both arms simultaneously.  The plumbing can be set up on a per-injection-port basis, allowing up to 8 different gradients and columns to be left on the system.  Direct control of onboard valves simplifies the control of the system.  Testing with multiple chromatographic setups, direct infusion and high-speed trap and elute experiments can be performed in a completely automated fashion.  Alternatively, if two different scientists are using the system, they can run independent experiments easily by each using a different arm.

All-in-One Software, Multiplexing, and Multi-Batch Automation 

The ADDA software was developed by Sound Analytics LLC. It offers excellent instrument control along with the ability to access MS/MS conditions from a central database.  Intuitive visual interfaces simplify sample setup and batch building.  Full multiplexing support is available to multiplex gradient runs.  The software effortlessly switches batches to different arms and different injection ports, allowing for automated overnight runs of many different batches of different chemistries without pause.   The runs can then be analyzed using the mass spectrometry software, making it easy to integrate all peaks and export them to Excel or another software system. 

Flexibility to perform 8-second trap and elute runs, or full gradient chromatography without compromise.

Unlike other high-speed systems, the ADDA system has the ability to perform high speed trap and elute runs while maintaining the ability to perform traditional full gradient chromatography assays.  Workflows can be designed that easily incorporate both types of analyses, remotely queuing each type of analysis to run on a different injection port.

Speed = $$

The ADDA system represents a significant leap forward in both speed and cost savings within high throughput drug discovery.  For instance, a lab performing 1000 samples at 1 min per run will spend 16 hours (+ setup time) running samples.  A similar lab, running at 10 seconds per sample in dual arm trap elute mode on the ADDA will turn the samples around in less than 3 hours, allowing for same-day turnaround for large sample volumes.  4000 samples can be analyzed with a single mass spectrometer in the course of an 8-6pm business day.  This can also translate into numerous benefits: less complicated pooling needed, fewer mass spectrometers used, happier customers, and faster drug development.

 Why 2 arms vs. 1 ?

• Speed & Time: One needle can be washed while the other needle is injecting into the sample loop

• Quality: One needle can be performing one batch while the other needle performs the next, remotely, without fear of crosscontamination.

• Redundancy: Redundant lines and arms can be used for failure recovery during overnight runs.

• Co-Working Harmony: One scientist can use and manage one side arm and it’s injection ports, while a second scientist can use and manage the other side, without interference or need to undo each other’s work.

Integration with DiscoveryQuant™ Software from AB SCIEX

A major benefit of the ADDA System is its ability to closely integrate with DiscoveryQuant™ Software from AB SCIEX.  This includes

• Full integration with the DiscoveryQuant™ Software compound database for optimized compound parameters on AB SCIEX Triple Quadruopole and QTRAP platforms

• Robust support for AB SCIEX mass spectrometers

• Ability to use DiscoveryQuant™ Software to analyze data produced using the ADDA and an AB SCIEX mass spectrometer.

Compatibility:

The ADDA System is compatible with standard pumps from Shimadzu and Agilent, as well as other major manufacturers.  Please contact Apricot Designs for more information.

Carryover:

The ADDA System’s unique 2-arm design allows for longer 2-solution internal and external needle washes without significant slowdown of the system, creating an adaptable low-carryover system.