利用荧光标记抗体与目的抗原的特异性结合，通过荧光显微镜对抗原处的荧光进行拍照 ，可知细胞中目的抗原的定位和表达情况。

技术原理

根据抗原抗体反应的原理，先将已知的抗原或抗体标记上荧光基团，利用这种荧光抗体（或抗原）作为探针检查细胞内的相应抗原（或抗体）。借助荧光显微镜观察荧光，从而确定细胞内抗原/抗体的性质,并对其进行定位。比较不同条件处理的实验组之间，细胞内蛋白的表达差异和细胞定位。

结果展示

目的细胞按实验设计组别进行药物处理后，收集细胞悬液，离心，用缓冲液洗涤，加细胞染色缓冲液重悬后，用PE标记的CD11b抗体进行染色，染色结束后，用细胞染色缓冲液洗涤、重悬，进行流式仪检测 （Millipore，Guava easyCyte HT），分析各实验组别中CD11b 阳性细胞百分比差异。

参考文献

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