随着基因组研究的迅速发展,全基因组测序已逐步成为微生物基础研究的重要手段之一。通过基于PacBio第三代测序和Illumina二代测序,根据菌株具体情况制定zei优策略,zei终得到一条完整的基因组序列(1 contig,0 gap),是细菌基因组测序的zei高要求。
技术路线:
技术方案:
结果案例:
细菌基因组完成图
细菌基因组甲基化图
技术指标:
参考文献:
1、Samina Mehnaz et al. Complete Genome Sequence of the Sugar Cane Endophyte Pseudomonas aurantiaca PB-St2, a Disease-SuppressiveBacterium with Antifungal Activity toward the Plant Pathogen Colletotrichum falcatum. Genome Announc.2014 vol. 2 no. 1 e01108-13
3、Bashir, et al. A hybrid approach for the automated finishing of bacterial genomes, Nature Biotechnology 2013, 30, 701707