产品描述
Agilent aCGH芯片检测
简介
拷贝数变异（包括DNA拷贝数增加（重复）、丢失（缺失）以及多等位基因或复杂重排）是基因组变异的重要原因之一，它与人类的很多疾病相关，如孟德尔遗传疾病；智力缺陷；孤独症；精神分裂症；肿瘤；自身免疫病等等。传统的染色体核型分析方法（如染色体区带染色分析，FISH等）可以检测染色体大范围结构变异。但这些方法具有实验操作繁琐，分辨率低，不能覆盖全基因组等缺点。基于基因芯片的比较基因组杂交技术（array-based Comparative Genomic Hybridization, aCGH）可以更加准确地发现疾病相关的染色体的变异，为疾病的诊断和治疗提供极大的帮助，这对高风险人群的健康水平筛查，疾病预防有重要意义。Agilent利用其先进的芯片生产工艺，开发出高性能的aCGH芯片，广泛用于基因的拷贝数检测和染色体结构变化检测。Agilent CGH芯片覆盖全基因组，在包括基因区域（内含子区、外显子区）、基因间区域以及对疾病研究极其重要的亚端粒区域（但不包含重复序列）的分布大致相同。
实验流程
技术优势
灵敏度和特异性：相比其它CGH芯片平台，具有极高的灵敏度和特异性，可有效检测单拷贝的失和扩增；
适用样品类型广：能够有效检测FFPE（Formalin-Fixed and Parrffin-Embedded，FFPE）样品；
数据处理软件强大：Agilent Genomic Workbench具有图形化显示，强大的数据统计功能；
可有效检测肿瘤发生过程中伴随的DNA拷贝数变化：如染色体的畸变；寻找新的原癌基因或抑癌基因；发现新的DNA拷贝数标记，用于临床诊断、疾病的分型等。
产品列表
物种 | 产品名称 | 产品编号/设计ID | 芯片格式 | 探针数量 | 探针间距(中位值) | 数据库版本 |
人Human | SurePrint G3 Human CGH Microarray 1×1M | G4447A /021529 | 1 x 1M | 963,029 | 2.1 KB overall median ; 1.8 KB in Refseq genes | UCSC hg18 (NCBI Build 36), March 2006 |
SurePrint G3 Human CGH Microarray 2x400K | G4448A /021850 | 2x400K | 411,056 | 5.3 KB overall median; 4.6 KB in Refseq genes | UCSC hg18 (NCBI Build 36), March 2006 | |
SurePrint G3 Human CGH Microarray 4x180K | G4449A /022060 | 4x180K | 170,334 | 13 KB overall median; 11 KB in Refseq genes | UCSC hg18 (NCBI Build 36), March 2006 | |
SurePrint G3 Human CGH Microarray 8×60K | G4450A /021924 | 8 x 60K | 55,077 | 41 KB overall median; 33 KB in Refseq genes | UCSC hg18 (NCBI Build 36), March 2006 | |
SurePrint HD Human CGH Microarray 1 x 244K | G4411B /014693 | 1 x 244K | 236,381 | 8.9 KB overall median; 7.4 KB in Refseq genes | UCSC hg18 (NCBI Build 36), March 2006 | |
SurePrint HD Human CGH Microarray 2×105K | G4412A /014698 | 2 x 105K | 99,026 | 22 KB overall median; 19 KB in Refseq genes | UCSC hg18 (NCBI Build 36), March 2006 | |
SurePrint HD Human CGH Microarray 4x44K | G4426B /014950 | 4 x 44K | 42,494 | 43 KB overall median; 24 KB in Refseq genes | UCSC hg18 (NCBI Build 36), March 2006 | |
小鼠Mouse | SurePrint G3 Mouse CGH Microarray 1x1M | G4838A /027414 | 1 x 1M | 963,261 | 1.8 KB overall median; 1.5 KB in Refseq genes | UCSC mm9 (NCBI Build 37), July 2007 |
SurePrint G3 Mouse CGH Microarray 4x180K | G4839A /027411 | 4x180K | 170,305 | 10.9 KB overall median; 9.1 KB in Refseq genes | UCSC mm9 (NCBI Build 37), July 2007 | |
SurePrint HD Mouse CGH Microarray 1 x 244K | G4415A /014695 | 1 x 244K | 235,402 | 7.8 KB overall median; 6.2 KB in Refseq genes | UCSC mm8 (NCBI Build 36), February 2006 | |
SurePrint HD Mouse CGH Microarray 2 x 105K | G4416A /014699 | 2 x 105K | 98,797 | 19.3 KB overall median; 15.7 KB in Refseq genes | UCSC mm8 (NCBI Build 36), February 2006 | |
大鼠 Rat | SurePrint G3 Rat CGH Microarray 1×1M | G4840A /027065 | 1 x 1M | 962,967 | 1.7 KB overall median, 1.3 KB in Refseq genes | UCSC rn4 (Baylor HGSC Build 3.4) November 2004 |
SurePrint G3 Rat CGH Microarray 4x180K | G4841A /027064 | 4x180K | 170,012 | 10.7 KB overall median, 8.0 KB in Refseq genes | UCSC rn4 (Baylor HGSC Build 3.4) November 2004 | |
SurePrint HD Rat CGH Microarray 1 x 244K | G4435A /015223 | 1 x 244K | 235,974 | 4.9 KB overall median, 4.7 KB in Refseq genes | UCSC rn4 (Baylor HGSC Build 3.4), November 2004 | |
SurePrint HD Rat CGH Microarray 2 x 105K | G4436A /015235 | 2 x 105K | 97,973 | 19.1 KB overall median, 11.3 KB in Refseq genes | UCSC rn4 (Baylor HGSC Build 3.4), November 2004 | |
牛 Bovine | SurePrint G3 Bovine CGH Microarray 4×180K | G4826A /025242 | 4 x 180K | 171,527 | 11.0 KB overall median | UCSC bosTau4 (October 2007) |
犬 Canine | SurePrint G3 Canine CGH Microarray 4×180K | G4826A /025522 | 4 x 180K | 171,534 | 2.7 kb overall median | UCSC canFam2 (May 2006) |
鸡 Chicken | SurePrint HD Chicken CGH Microarray 1 x 244K | G4423B /019553 | 1 x 244K | 235,980 | 4 KB overall median | UCSC galGal3 (WUSTL Build 2.1) May 2006) |
黑猩猩Chimpanzee | SurePrint G3 Chimpanzee CGH Microarray 4×180K | G4826A /024422 | 4 x 180K | 171,524 | 12.7 kb overall median | UCSC panTro2 (March 2006) |
水稻 Rice | SurePrint G3 Rice CGH Microarray 4×180K | G4826A /025843 | 4 x 180K | 171,861 | 14.9 kb overall median | RGSP Build5 (June 2008) |
恒河猴 Rhesus Macaque | SurePrint G3 Rhesus Macaque CGH 4×180K | G4826A /025843 | 4 x 180K | 171,495 | 14.9 kb overall median | UCSC rheMac2 (January 2006) |
