“英拜为您实验加速”
技术服务网址：http://www.yingbio.com/
服务热线************br>邮箱：daihp@yingbio.com 、 huizhang1228@foxmail.com

Melanoma EpiTect Methyl qPCR Array

黑色素瘤甲基化PCR芯片
 

ProductSpeciesTechnologyCat. No.Melanoma EpiTect Methyl qPCR ArrayHumanDNA MethylationEAHS-081ZMelanoma EpiTect Methyl qPCR ArrayMouseDNA MethylationEAMM-081ZMelanoma EpiTect Methyl qPCR ArrayRatDNA MethylationEARN-081Z

The Human Melanoma EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 genes showing hypo- or hypermethylation in melanoma. Melanoma accounts for less than 5% of skin cancers, but 80% of the deaths, illustrating the aggressiveness and malignancy of the disease. Epigenetic studies of dysregulated and hypermethylated tumor suppressor genes have identified novel therapeutic targets for melanoma. This array includes genes frequently observed by genomewide analyses to be hypermethylated in melanoma, as well as genes involved in melanoma onset and progression. Profiling cellular or fresh tissue genomic DNA samples with these arrays may help correlate CpG island methylation status with biological phenotypes. The results may also help provide further insights into the molecular mechanisms and biological pathways behind oncogenesis and cancer biology. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the promoter methylation status of 22 different genes involved in melanoma initiation and progression with this DNA methylation PCR array.
The EpiTect Methyl II PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease
 
黑色素瘤甲基化PCR芯片用于研究在黑色素瘤中低甲基化和高甲基化的22个基因的启动子甲基化状态。黑色素瘤占皮肤癌不到5%，但80%的人死亡,说明疾病的侵略性和恶性。表观遗传研究低甲基化和高甲基化肿瘤抑制基因鉴定了黑色素瘤新的治疗靶标。这个芯片上的基因包括全基因组分析发现在黑色素瘤中经常发生高甲基化的基因，以及参与黑色素瘤发生和进程的基因。利用这些芯片分析细胞或新鲜组织基因组DNA样本有助于关联CpG岛甲基化状态与生物表型。结果还可以洞察肿瘤发生和进程,及其病理背后的分子机制和生物学通路。利用这个芯片，通过简单的限制性内切酶消化和实时定量PCR，就可以研究分析22个参与黑色素瘤起始和进程的不同基因的启动子甲基化状态。
 
提供96-孔板和384-孔板( 4 X 96 )。
Signaling Pathways Dysregulated in Melanoma: GDF15 (PLAB), RB1.
Cellular Stress Responses:
Drug & Chemical Response: CDH3, DNAJC15, LRP2, SOCS3.
Immune: DPP4, IRF8, PRDX2, THBD.
Hypoxia: AKAP12, DPP4, SOCS3.
Oxidative: PRDX2, TPM1.
Cell Growth & Proliferation: AKAP12, DPP4, LRP2, PPP2R4, PRDX2, RB1, SOCS2.
Extracellular Matrix & Cell Adhesion: ADAMTS18, CDH3, DPP4, ENC1, MME, QPCT, SOCS2, SOCS3, TPM1.
Apoptosis Regulation: PRDX2, SOCS2, SOCS3, TNFRSF10A, TP53INP1.
Transcription Factors & Cofactors: IRF8, RB1, TNFRSF10A.
Others: PPP1R3C, RASEF.