产品描述
外泌体计数、粒径、蛋白表达、蛋白共定位一次完成
检测样本类型 | 对细胞培养上清、血浆、血清、尿液、脑脊液、唾液等生物样本中的外泌体直接进行分析 |
捕获抗体种类 | anti-CD81, anti-CD9, anti-CD63, 同型IgG对照；可自定义 |
单次上样体积 | 35 µl稀释样本 |
重复检测数目 | 3复孔 |
荧光抗体种类 | CD9（Blue）/ CD81（Green）/ CD63（Red） |
实验原理
① 35 μL外泌体样品滴加在芯片上孵育；
② 预先包被的抗体特异结合外泌体表面蛋白以捕获外泌体；
③ 再使用荧光抗体特异性标记需要表征的标记物；
④ 后用ExoView R100检测外泌体粒径、计数、蛋白表达（CD9，CD81，CD63等）及共定位。
检测流程
产品类别
产品货号 | 产品名称 |
EV-TETRA-C | 人外泌体检测试剂盒 |
EV-TETRA-P | 人血浆外泌体检测试剂盒 |
EV-TETRA-M2 | 鼠外泌体检测试剂盒 |
EV-TETRA-C-CAR | 人外泌体内容物检测试剂盒 |
EV-TETRA-P-CAR | 人血浆外泌体内容物检测试剂盒 |
EV-TC-FLEX | 自由捕获人外泌体检测试剂盒 |
EV-TP-FLEX | 自由捕获人血浆外泌体检测试剂盒 |
EV-TC-FLEX-CAR | 自由捕获人外泌体内容物检测试剂盒 |
EV-TP-FLEX-CAR | 自由捕获人血浆外泌体内容物检测试剂盒 |
EV-TM-FLEX | 自由捕获鼠外泌体检测试剂盒 |
EV-TM-FLEX-CAR | 自由捕获鼠外泌体内容物检测试剂盒 |
EV-FLEX-2 | 自由捕获外泌体检测试剂盒 |
EV-FLEX-2 -CAR | 自由捕获外泌体内容物检测试剂盒 |
EV-CTETRA-1/2/3 | 人外泌体检测试剂盒+1/2/3个自定义捕获抗体 |
EV-CTETRA-1/2/3-CAR | 人外泌体内容物检测试剂盒+1/2/3个自定义捕获抗体 |
EV-CUST-1/2/3/4/5/6 | 自定义1/2/3/4/5/6抗体捕获外泌体检测试剂盒 |
EV-CUST-1/2/3/4/5/6-CAR | 自定义1/2/3/4/5/6抗体捕获外泌体内容物检测试剂盒 |
试剂盒特点
特异性捕获 芯片上可包被多达6种捕获抗体，特异性捕获含特定蛋白标记物的外泌体。 |
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绝对阳性外泌体计数 芯片捕获外泌体后，可通过SP-IRIS技术直接检测样品中外泌体的数量。 |
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单个外泌体蛋白共定位分析 检测每个外泌体的荧光信号并进行统计，可获得荧光共定位信息，用于分析样品中不同表型外泌体的比例（如右图所示）。 |
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无需纯化 使用抗体捕获模式，防止样品中杂质影响结果，可直接检测血液、尿液和细胞培养液中的外泌体，未纯化样品的测量结果与纯化后基本一致（如右图所示）。 |
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粒径分辨率高 高精度SP-IRIS技术，可检测≥50 nm的外泌体，测量结果与电子显微镜检测结果基本一致，并统计生成外泌体的粒径分布结果（如右图所示）。 |
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可检测外泌体内容物 试剂盒配套相应的穿膜剂，可穿透外泌体并对外泌体内容物进行染色并检测，未穿膜时只能检测到跨膜蛋白CD9的荧光信号，穿膜后即可检测到外泌体内容物Syntenin的表达（如右图所示）。 |
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测试数据
外泌体荧光数量统计
外泌体粒径检测
外泌体内容物检测
荧光强度与粒径关系
荧光共定位分析
发表文章
• Andras Saftics.(2021) Data evaluation for surface-sensitive label-free methods to obtain real-time kinetic and structural information of thin films: A practical review with related software packages. Advances in Colloid and Interface Science.
• Kyoung-Won Ko.(2021) Integrated Bioactive Scaffold with Polydeoxyribonucleotide and Stem-Cell-Derived Extracellular Vesicles for Kidney Regeneration. ACS Nano.
• Tanina Arab. (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms. Journal of Extracellular Vesicles.
• Niaz Z.Khan.(2021) Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, Behavior, and Immunity.
• Dario Brambilla. (2021) EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles. Analytical Chemistry.
• Enkhtuya Radna. (2021) Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth. Lab on a Chip.
• Li, M., Soder. (2021) WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1. Journal of Extracellular Vesicles.
• Crescitelli, R. (2021) Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols.
• Berger, A. (2021). Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula. Nanoscale.
• Vidal, M. (2020) Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids. Advanced drug delivery reviews.
• K Cho, H Kook.(2020)Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. Journal of Extracellular Vesicles.
• Maximillian A. Rogers.(2020)Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Cell Biology.
• Annette M. Marleau.(2020)Targeting tumor-derived exosomes using a lectin affinity hemofiltration device. Cancer Research.
• Alessandro Gori.(2020)Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis. Journal of Extracellular Vesicles.
• Rossella Crescitelli.(2020)Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles.
• Maria S. Panagopoulou.(2020) Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence. Journal of Extracellular Vesicles.
• WeiYan.(2020) Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle. Trends in Cancer.
• Daniel Bachurski. (2019) Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell.
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