1.Array Product Portfolio Cytokine Antibody Arrays for Human, Mouse and Rat samples Quantitative and semi-quantitative ELISA Sandwich and Label-based
Phosphorylation Antibody Arrays RTK and EGFR
Antibody Array Analysis software Protein Arrays
2.Array using protein as a capture molecule Recombinant/Purified Protein Antibody Peptide arrays Reversed phase (biological samples) Transcription factor Tissue arrays
3.Types of Cytokine Array Targets
Cytokines Inflammatory factors (interleukins, etc.) Chemokines Angiogenesis Atherosclerosis MMPs Growth Factors Adipokines Soluble receptor Signal transduction (Phosphorylation) Broad Screen (all of the above)
4.全球领先优势:
(1)Global analysis of gene or protein expression (2)High-throughput assays (3)Substantially reduced costs (4)Small sample volume
5.Antibody Arrays vs. Mass Spec
(1)“Unbiased” = abundant Nanogram/ml vs. picogram/ml sensitivity (2)Cheaper per sample (3)Faster throughput (4)Speeds up discovery process: Discovery Validation Application All in one platform
6.Antibody Arrays vs. Luminex
(1)Affixing capture Ab reduces interactions (2)Can screen hundreds of proteins (3)More flexibility Equipment Mode of detection (Fluor, Chemi, Color) (4)Less equipment maintenance
7.cDNA Arrays vs. Antibody Arrays
(1)cDNA arrays measure only RNA levels (2)↑ RNA ≠↑protein levels or function (3)Protein functions are modulated by Interactions with other proteins, DNA and/or RNA Biochemical modification of individual amino acids (4)Antibody arrays measure protein levels Can demonstrate patterns of coupled protein expression Can detect biochemical modifications of protein Can be used to detect protein-protein, protein-DNA or protein-RNA interactions Results are more biologically meaningful
9.Features of Cytokine Antibody Array (membrane-based):
(1)Detection Up to 274 cytokines in one experiment. Detection in picogram(pg) range. (2)Highly specific, little cross-reactivity (3)Handles a wide range of samples Conditioned media, plasma, serum , urine and other body fluids Cell or tissue lysates (4)Requires low sample volumes As little as 100 μLof sample (5)Easy to use, requires no specialized equipment (6)Cost-effective for routine use
10.Advantages of Cytokine Array System: G-Series
(1)Requires laser fluorescence scanner (2)Sensitivity similar to membrane arrays (3)Greater dynamic range (4)Cheaper per sample than membranes (5)High-throughput (100’s of samples/day) (6)Small sample volume (10-50 μL/array)