应用领域:多组学/蛋白质组/代谢组/脂质组,多组学/蛋白质组/代谢组/脂质组
检测样品:Spirulina Platensis (SP6)
检测项目:质谱
参考标准:、
Elucidation of metabolic changes in HFD-ApoE–/– model by SP6 peptide: A flow injection analysis magnetic resonance mass spectrometry (FIA-MRMS) study
Natural peptides have emerged as an attractive option for the treatment of cardiovascular diseases. A novel peptide from Spirulina Platensis (SP6) was evaluated for its potential anti-atherosclerotic effect in a high fat diet ApoE−/− mice model using a metabolomics approach.
Abstract
The employment of natural peptides in the treatment of cardiovascular diseases is an attractive option to pharmacological therapy. In this study, a novel antihypertensive peptide from Spirulina Platensis (SP6) was employed to evaluate its potential anti-atherosclerotic effect in high fat diet ApoE–/– mice model.
Untargeted metabolomics and lipidomics was performed by a Flow Injection Analysis Magnetic Resonance Mass Spectrometry (FIA-MRMS) approach, resulting in high mass resolution and mass accuracy as well as repeatability and fast analysis time. Results showed a distinct metabolic switch, with the positive modulation of several key markers of atherosclerosis progression, such as sphingolipids and glycerophospholipids, amino acids and tricarboxylic acid (TCA) cycle intermediates. The FIA-MRMS approach has been proven to be a fast and effective tool for large scale investigation of SP6 effects in large cohorts.