To demonstrate successful separation and detection of 2-aminobenzamide (2-AB)-labeled complex glycan libraries on a Thermo Scientific™ Vanquish™ Flex UHPLC system equipped with a Vanquish Fluorescence Detector F. Retention time precision for these glycan libraries as well as for a 2-AB-labeled single glycan standard are reported. Area precision and linearity are evaluated for three labeled glycan standards. Further, recommendations for the selection of the right detector flow cell are given.