Environmental pollution caused by a variety of chemicals is drawing attention to the need for a more efficient method of simultaneous multicomponent analysis. One of the most effective methods of comprehensive, simultaneous multi-component analysis of semi-volatile organic compounds (SVOC) is by GC-MS in conjunction with an Automated Identification and Quantification System with a Database (AIQS-DB). As a comprehensive SVOC extraction method for environmental water samples using AIQS-DB, liquid-liquid extraction with dichloromethane has typically been used. In this research, however, we investigated the use of solid-phase SVOC extraction, which requires the use of less solvent. Here, an analytical method developed for comprehensive analysis of SVOCs in water samples using AIQS-DB is reported.