The measurement of lipids in a biological

matrix to

study

their responses to

diseases, drugs, genetic modifications,

and other stimuli requires a Q

-

TOF

system with outstanding resolution,

mass accuracy, and sensitivity.

In this application, the 6545 Q

-

TOF

and 1290 Infinity LC system are used

to analyze an induced sputum lipid extract

from a

donor

with chronic obstructive

pulmonary disease (COPD). T

he 6545

Q

-

TOF is tuned by novel Swarm Autotune

for optimal performance in the m/z range

of the lipids, and data acquired in both

positive and negative ionization modes

with reference mass correction