This Application Note describes a UHPLC/Q

-

TOF/MS method for the detection

of 240 pesticides and pesticide metabolites in diffi cult food matrixes using

the Agilent All Ions MS/MS technique. The method benefi ts from increased

chromatographic resolution using the Agilent 1290 Infi nity UHPLC system and

improved ionization capa

bilities with Agilent Jet Stream ionization source. More

importantly, the successful screening and quantitation are accomplished by the

innate sensitivity improvement of the Agilent 6545 Q

-

TOF LC/MS system and

ion transmission tuning to facilitate the o

ptimal ion transmission of small fragile

organic molecules. The All Ions MS/MS technique features the easy acquisition

method setup and retrospective data interrogation with expanded library. Black

tea matrix was chosen for its complexity, and avocado m

atrix was chosen to

represent food commodities with high lipid content.

Our results demonstrate that the improved detection of small organic molecules

by the 6545 Q

-

TOF LC/MS system allows the screening and quantitation of most

of the targeted pesticid

es below the maximum residue limits (MRLs) specifi ed by

regulations