This study investigated the feasibility of automating an established cell surface

protein isolation protocol using the Agilent AssayMAP Bravo automation platform.

Epidermoid cancer cell lines

-

A431 was used as an

in

-

vitro

model system, and

cell surface proteins were isolated using a combination of AssayMAP Bravo

automation and a Pier

ce Cell Surface Protein Isolation kit. The isolated proteins

were then analyzed using the Agilent 2200 TapeStation system in conjunction

with the Agilent P200 ScreenTape assay for differential electrophoresis profi le.

The eluted proteins were then anal

yzed using the Agilent HPLC

-

chip coupled to

an Agilent 6550 iFunnel Q

-

TOF with dual stage ion funnel technology. Key cell

surface proteins were identifi ed including extracellular matrix proteins, moderately

abundant proteins including pancreatic marker

protein, plectin

-

1 along with F

-

box

leucine rich repeat protein

-

2,

beta

-

actin, and PGK

-

2. This study demonstrates

that the AssayMAP Bravo platform can be conveniently used for automated

high

-

throughput sample preparation workfl ows involving affi nit

y purifi cations