Biochips functionalized with Cystamine/Glutaraldehyde layers allow a good reproducibility between the anti-ovalbumin spots within the same chip. Moreover, this surface chemistry developed by Genoptics is an easy way to immobilize receptors on a biochip surface. No modification of the immobilized biomolecule is needed and we have shown that a hundred successive interaction/regeneration process can be tolerated without loss of activity.