Comparison of the simple triexponential deconvolution with the freeform MEM shows that they are qualitatively similar for HSA-Q binding. The MEM is more sensitive to environmental and structural changes in the complex. The HORIBA Jobin Yvon 5000U lifetime spectrofluorometer with TCSPC is an integral part of researching structure-property relations during pro-tein-binding studies for the biochemical and medical fields.