简介：To develop a highly sensitive, selective, accurate and precise method for absolute quantitation of endogenous salivary peptides on a triple quadrupole mass spectrometer by using isotopically labeled peptides as internal standards.

　　仪器：TSQ Vantage

　　结论：

　　1. A 46-transitions H-SRM assay on a new TSQ Vantage triple quadrupole mass spectrometer was used to successfully determine the absolute amounts of six targeted endogenous peptides from parotid and sublingual/ submandibular gland secretions of eight human donors using the AQUA strategy without any prior peptide enrichment or sample clean up.

　　2. The H-SRM assay was highly sensitive and enabled to detect the native salivary peptides at less than 10 attomole/μL level.

　　3. The analytical precision of the H-SRM assay was good. The RSDs of five peptides were less than 12% (average 8.5%). The RSD of the low-level peptide AAPDEKVLDSGFR was also within acceptable range (25%).

　　4. Significant abundance variations for each targeted endogenous peptide were observed between donors and between glandular sources. The biological meaning for these variations is a subject of further investigation.