简介：The goal of the research reported in this application note was to investigate the effects of stepped HCD collision energy on relative quantitation of TMT-labeled peptides.

　　仪器：LTQ Orbitrap HCD

　　结论：

　　• A single-value, optimized collision energy can produce good identification and reporter (TMT)-based quantitation results for most peptides.

　　• For peptides that do not yield adequate quantitation results at a fixed CE, a stepped CE method can be used.

　　• Stepped CE showed overall improvement of quantitation results including the number of quantified peptides and reproducibility of quantitation.

　　• Increasing the number of microscans for a single-value, optimized collision energy improved the quality of spectra, but it did not significantly