Orbitally shaken, single-use bags represent a new method for the production

of seed cultures or protein production on the lab scale.

Using this new technology, successful cultivation of the cell lines CHO XM 111

(Chinese Hamster Ovary) and Sf-21 (Sf-9) (Spodoptera frugipedra) was accomplished.

Also, scale-up of the CHO cell cultivation from 1 L to 10 L working volume

is possible using orbital-shaken, single-use bags. For these cell cultures

a serum- and protein-free chemically defined media was used, which needs

sophisticated system efforts for the cell growth, but is a requirement for the

production of therapeutic products.