前言：

　　In the clinical research setting, quantitative measurements of estrone (E1) and estradiol (E2) in serum typically have been done with immunoassay or liquid chromatographytandem mass spectrometry (LC-MS/MS). LC-MS/MS is preferred over immunoassay and other analytical techniques because of its high sensitivity. E1 and E2 are usually chemically derivatized before they are detected by mass spectrometry for enhanced sensitivity. The derivatization step extends the sample preparation procedure and usually involves chemicals/reagents that might compromise the performance of the mass spectrometer in the long term.

仪器：

Thermo Scientific TSQ Quantum mass spectrometer

结论：

　　We have developed and fully validated a simple, fast and sensitive LC-APCI-MS/MS method for measurement of E1 and E2 in serum/plasma without derivatization. The LLOQ for E1 and E2 are 3.5 and 4.4 pg/mL, respectively. The method was linear between 3.5 and 1019.3 pg/mL for E1, and 4.4 and 1032.5 pg/mL for E2. No ion suppression or carryover was observed. In addition, for clinical research laboratories, this method offers high precision and recovery.