The Q Exactive Focus MS in combination with an UltiMate 3000 HPLC was used to create a variable data-independent acquisition method for the detection and quantification of 44 multiclass veterinary drug residues. This novel method proved to have the required LOD sensitivity, and ability to confirm identity using retention time, accurate m/z, isotopic ratio, and fragment ions to exceed the EU regulatory requirements (EC/657/2002). The vDIA approach extends confirmation options for non-targeted and unknown screening approaches, while maintaining a high level of sensitivity and selectivity. All data processing was accomplished with the easy-to-use TraceFinder software both for identification and all stages of identity confirmation. vDIA accomplishes the goal of being an accurate and sensitive analysis for the detection of non-targeted compounds(i.e. those from the 44 compound panel) and also for the screening of unknown compounds post-analysis.