LC-MS analyses were performed on a IdeS digest of a NIST monoclonal antibody (mAb) reference materials.

　　Spectral accuracy was evaluated under different data acquisition conditions to ensure high spectral accuracy can be achieved for collecting digested NIST mAb.

　　Through peak shape calibration and spectral accuracy calculation, relative quantitation of computer simulated deamidated light chain subunits of mAb was performed.