Day 0One frozen vial of Murine Embryonic Fibroblasts (MEFs) is thawed quickly in a 37oC water bath
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P
Collect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0
Collagen gel containing 3T3 fibroblasts (dermal equivalent for raft culture)Ingredients for 6 x collagen matrices in a 6-well plate;Roughly 3x106 J2-3T3s (a fully confluent T75?)1
Chemotherapy remains the mainstay in the treatment and management of many cancers