细胞/组织裂解液
溶液准备 2×样品缓冲液:130mM Tris-HCl ( pH8.0 ) , 20% ( v/v ) 甘油 , 4.6% ( w/v ) SDS , 0.02% 溴酚蓝 ,2%DTTPBS ( pH7.4 ) :10mM Na2HPO4,1.8mM KH2PO4 ,50mM NaCl,2.7mM KClRIPA缓冲液:50mM Tris-HCl(pH7.4),150mM NaCl,1mM EDTA,1%Triton×100 ,1%去氧胆酸钠,0.1%SDS,1mM PMSF,1μg/ml 牛胰蛋白酶抑制剂 ( aprotinin ),1μg/ml亮抑蛋白酶肽 ( leupeptin )裂解液缓冲液:10mM Tris-HCl(pH7.4),0.15M NaCl,5mM EDTA(pH8.0),1%Triton×100;使用前加5mM DTT,0.1mM PMSF异丙醇和5mM ε-氨基己酸