Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at ...
完整的实验方法请点击下面的链接下载: ...
1.Restrict DNA and gel purify Cut 100ug DNA removing as much plasmid sequence as possible from the insert. Run on an agarose gel. Cut out band and electroelute in 0.5X TBE. Add an equal volume of ...
The following is an outline of the method that I use to manufacture injection needles for ES cell injection. I use high quality borosilicate glass obtained from World Precision Instruments (see vendor ...
The purity of the transgene DNA used for microinjection is critical for the successful production of transgenic founder mice. DNA impurities in the form of bacterial endotoxins or organic contaminants ...