Culture should be 2 days old and colonies should be of medium to large size. This is important as cells need to be in log growth phase. Two small (25cm2) flasks are required one is harvested in morni ...
Transfer Pipettes Glass used for making transfer pipettes : GC120-15 (350 pieces) by Clark Electromedical Instruments. OUTSIDE DIAMETER: 1.2mm INSIDE DIAMETER: 0.69mm LENGTH: 150mm Agent in Aust ...
Solutions 500mM K4Fe(CN)6.3H2O (Potassium Ferrocyanide) (Sigma: P9387) in DDW 500mM K3Fe(CN)6 (Potassium Ferricyanide) in DDW Solution A : 0.2M NaH2PO4.H2O (27.6g/l) Solution B : 0.2M Na2HPO4 (284 ...
PCR screens must be designed to detect transgene DNA at the single copy level. Southern Blots analysis of transgenic mice need copy standards to estimate copy number. Copy standards are prepared by ...
Attractene Transfection Reagent高效转染各种贴壁真核细胞♦ 高效DNA转染,极低细胞毒性♦ 快速、简便的操作流程♦ 所有贴壁细胞 (包括难转细胞)的最佳选择♦ 非常适用于瞬时转染,稳定转染,共转染和 shRNA载体转染♦ 不含来自动物的组分更高效率转染质粒DNAAttractene 试剂采用非 ...