These protocols have come from the transgenic mouse list and are intended as a guide none of them have been tried in this lab Cell(s) transferred in small volume of water to a .5 ml tube and lysed by ...
1、 选取7~8周龄雌性小鼠,阴道口封闭,作为供体,下午3:00左右,每只小鼠腹腔注射PMSG(10 IU)。 2、 47~48小时后,每只小鼠腹腔注射HCG(0.8 IU),并与正常公鼠合笼;另取数只适龄母鼠(2月龄以上)作为受体,阴道口潮红,与结扎公鼠合笼。 3、第二天上午9:00前观察供体、受体,有精栓者拿出备用。受体笼拿出作好隔离措施。 4、10:30左右,断颈处死供体,手术取出整个 ...
Before you start have ready the following: - Sterilize 2 pairs of curved forceps 1 pair of iris scissors and 2 pairs of fine watchmaker forceps. - 6ml syringe filled with DMEM with HEPES with an 18 ...
Pre-sterilize two pairs of fine forceps one pair iris scissors and one blunt-ended 23 gauge needle. Take 3.5 day pregnant mouse kill and dissect out entire uterus using sterile technique. Place uteru ...
Blastocyst transfer is usually performed 24 hours after aggregation when the morulae have become expanded blastocysts and on the same day as injection. A little time is given between injection and tra ...