Buffer composition is 10 mM Tris-HCl pH 7.5 0.1 mM EDTA 30 microM spermine 70 microM spermidine 100 mM NaCl. This buffer is more likely to produce transgenic mice with intact unfragmented DNA molecule ...
Mouse embryos staining including: in vitro transcription of riboprobe embryo fixation and permeabilization hybridization and post-hybridization washes immunodetection and development of signal. 完整的实验 ...
(from Hogan et al. Manipulating the Mouse Embryo Cold Spring Harbor 1994) Day 11. Dissect embryos and place each yolk sac in a 1.5 ml microfuge tube (can be frozen at -20°C prior to extraction). ...
The manipulation of embryonic stem (ES) cells to generate targeted mutations via homologous recombination has proved an invaluable resource for researchers from fields as diverse as embryology immunol ...
1. Purify plasmid from bacteria. We recommend the Qiagen EndoFree Plasmid Maxi kit for the purification of the targeting vector plasmid from bacteria. Please follow the directions in the kit. Electro ...