Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at 6 ...
1. Dissect embryos and place each yolk sac in a microfuge tube (can be frozen at -20°C prior to extraction). Original method cited for mouse toes or 2 mm tail Reagents: A) 25 mM NaOH / 0.2 mM ED ...
Blastocyst transfer is best performed after allowing injected embryos a little recovery time in culture. This allows better evaluation of the cells' survival - the embryos should recavitate if they ha ...
2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control.Replace 1X PBS solution over biopsy with 2 ...
1. Obtain the last 2mm of tail and place directly into 200ul 1X PCR Buffer with Nonionic Detergents (PBND) in a 1.5ml microfuge tube. (Tails can be stored at frozen in PBS or PBND until use.) 2. Add ...