Based on Krapp at al (1993) Plant Journal 3:817 with modifications. Comments or Questions? Send to me at: chunming.liu@wur.nl ------------------------------------------------------------------------ ...
Solutions: Extraction buffer: 200 mM Tris-HCl pH 7.5 250 mM NaCl 25 mM EDTA 0.5% SDS Arial He ...
1.protocol(1)collect piece of tissue (e.g. pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH(2)put in boiling H2Ofor 30 sec (optimum may need to be determined for each type ...
This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure with a variety of Populus species as well as tobacco and ...
day 1 1. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr Koncz & Schell) in YEP in 250 mL baffle flasks. 2. Grow at 28 °C shaking. 3. Prepare: ~ 3.5 L sterile H2 ...