Polymerase III in vitro TranscriptionSteve Hahnlast modified 10/15/99For the following reactions, us
in vitro TranscriptionReaction1 µl 10X Transcription Buffer (Ambion) 1 µl 10X NTPs (4 mM ATP, CTP, 1
Overview With this protocol, transcripts that were initiated from specific genes by RNA polymera
Transcription of cRNA probeReagents/SolutionsDNA (PCR or plasmid) with bacteriophage RNA polymerase
Polymerase III in vitro Transcription Steve HahnFor the following reactions, use appropriate shieldi