产地：MBI
货号：ER0971 Conditions for 100% Activity
1X Buffer Tango™:
33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA.
Incubate at 37°C.
Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Ligation and Recleavage
After 50-fold overdigestion with NheI, more than 95% of DNA fragments can be ligated and recut.
Storage Buffer
NheI is supplied in:
10 mM Tris-HCl (pH 8.0 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
ER0971 |
500 u (10 u/µl) |
1.00 ml |
ER0971 |
|
ER0972 |
2500 u (10 u/µl) |
1.00 ml |
ER0972 |
|
ER0975 |
1000 u (10 u/µl) |
1.00 ml |
ER0975 |
|
