产地：MBI
货号：ER1101 Conditions for 100% Activity
1X Buffer B:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA.
Incubate at 37°C.
Ligation and Recleavage
After 50-fold overdigestion with HphI, more than 70% of the DNA fragments can be ligated and more than 90% of these can be recut.
Storage Buffer
HphI is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
ER1101 |
300 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER1101 |
|
ER1102 |
1500 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER1102 |
|
