ER1141 |
400 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER1141 |
|
ER1142 |
2000 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER1142 |
|
产地：MBI
货号：ER1142 Conditions for 100% Activity
1X Buffer R:
10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl and 0.1 mg/ml BSA.
Incubate at 65°C. To ensure higher efficiency of digestion, perform the cleavage reaction under paraffin oil.
Ligation and Recleavage
After 50-fold overdigestion with TaiI, more than 95% of DNA fragments can be ligated and recut.
Storage Buffer
TaiI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
