产地：MBI
货号：ER1501
Conditions for 100% Activity
1X Buffer BfuI:
50 mM Tris-acetate (pH 7.9 at 37°C), 15 mM Mg-acetate, 100 mM K-acetate and 0.1 mg/ml BSA.
Incubate at 37°C.
Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Storage Buffer
BfuI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 5-fold overdigestion with BfuI, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. More than 90% of these can be recut.
