产地：MBI
货号：ER1931 Conditions for 100% Activity
1X Buffer Tango™:
33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA.
Incubate at 37°C.
Ligation and Recleavage
After 50-fold overdigestion with LguI, more than 90% of the DNA fragments can be ligated and recut.
Storage Buffer
LguI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
| ER1931 | 100 u (5 u/µl) | 1.00 ml | ER1931 | |
| ER1932 | 500 u (5 u/µl) | 1.00 ml | ER1932 | |
