AMVReverseTranscriptase,10u/ul-分析测试百科网

产品描述

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids (1). It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg²⁺ or Mn²⁺.

Cat No	Size	Price($)

A1201-200	200 units	75

A1201-500	500 units	155

The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

Applications

- First- and second-strand synthesis of cDNA.

- Primer extensions and RNA sequencing

- RT-PCR(a). Up to 10μl of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase.