产地：oxoid
货号：SR0103E
Description
Pseudomonas Agar Base is designed so that by the addition of the appropriate supplement (SR0102 or SR0103) the medium becomes selective for Pseudomonas aeruginosa or Pseudomonas spp. generally. The base medium is a modification of King’s A Medium1 in which magnesium chloride and potassium sulphate are present to enhance pigment production.
Pseudomonas CN Supplement (SR0102) is recommended for the selective isolation of Pseudomonas aeruginosa. The formula of the supplement was described by Goto and Enomoto2 following the demonstration of cetrimide as a selective agent by Lowbury and Collins3. Goto and Enomoto showed that addition of nalidixic acid at 15µg/ml, while at the same time reducing the cetrimide content to 200mg, improved performance. The medium gave better recovery of Pseudomonas aeruginosa with enhanced pigment formation whilst strongly suppressing Klebsiella, Proteus and Providencia spp., the latter organisms being the troublesome contaminants of conventional cetrimide media.
Pseudomonas CFC Supplement (SR0103) is recommended for the selective isolation of Pseudomonas spp. generally. Mead and Adams4 showed that reducing the cetrimide content to 10mg/ml allowed the growth of all pigmented and non- pigmented psychrophilic pseudomonads. To improve its selective action they added cephaloridine 50µg/ml) and fucidin (10µg/ml). This combination of agents gave a new and more specific medium for isolating pseudomonads from chilled foods and processing plants. Incubation should be carried out at 25-30°C for 48 hours.
Considerable importance is given to detection of Burkholderia cepacia (formerly Pseudomonas cepacia) in water systems, particularly where the water is to be used for the preparation of pharmaceuticals and cosmetics5. The organism is resistant to many commonly-used disinfectants. Burkholderia cepacia has emerged as an important opportunistic pathogen in urinary, abdominal, respiratory and other infections.
