产地:MBI
货号:EK0032
Description
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP T4 Polynucleotide Kinase exhibits 5'-phosphatase activity and catalyzes the exchange of phosphate group between 5'-P-oligo-polynucleotides and ATP (exchange reaction) (1).
The enzyme is also a 3'-phosphatase (2).