产地：MBI
货号：ER0011 Storage Buffer
AluI is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton X-100, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with AluI, approximately 95% of the DNA fragments can be ligated and recut.
Isoschizomers
Search for commercial isoschizomers using REsearch™.
Double Digestion
Perform double digestion using DoubleDigest™.
ER0011 |
600 u (10 u/µl) |
1.00 ml |
ER0011 |
|
ER0012 |
3000 u (10 u/µl) |
2 x 1.00 ml |
ER0012 |
|
