产地:MBI
货号:ER0311 Conditions for 100% Activity
1X Buffer R:
10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
Eco47I is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with Eco47I, more than 95% of the DNA fragments can be ligated and recut.
ER0311 |
800 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER0311 |
|
ER0312 |
4000 u (10 u/µl) |
2 x 1.00 ml |
1.00 ml |
ER0312 |
|